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Reconstitution · Dosing · Storage

The Protocol Guide

Everything you need to prepare, measure and store research peptides correctly. Read this before your first reconstitution.

01 Supplies 02 Mixing 03 Calculator 04 Dosing 05 Storage
01

What you'll need

Before you begin, gather your supplies and work on a clean surface. Everything that touches the vial should be sterile.

  • Your lyophilised (freeze-dried) peptide vial
  • Bacteriostatic water — the sterile solvent (available in our shop)
  • Alcohol swabs to sanitise both rubber stoppers
  • A reconstitution syringe (1–3ml) to draw the water
  • Insulin syringes (U-100) for measuring research doses
02

Reconstituting (mixing)

Reconstitution simply means adding liquid to the powder so it can be measured accurately. Take it slowly — peptides are delicate.

  • Swab the rubber stoppers of both the peptide vial and the bacteriostatic water.
  • Draw your chosen volume of bacteriostatic water into the reconstitution syringe (see the calculator below to decide how much).
  • Insert the needle into the peptide vial at an angle and let the water run slowly down the inside glass wall — never squirt it directly onto the powder.
  • Do not shake. Gently swirl or roll the vial until the powder fully dissolves into a clear solution.
  • If any particles remain or the solution looks cloudy, let it sit a few minutes and swirl again.

Reconstitution Calculator

Enter your vial strength, how much water you'll add, and your target research dose. We'll show the concentration and how many units to draw on a U-100 insulin syringe.

—Concentration
—Draw on U-100 syringe

On a U-100 insulin syringe, 100 units = 1ml. This tool is provided for laboratory calculation only and is not dosing advice.

03

Measuring a dose

Once reconstituted, the concentration is fixed — the same solution can yield different doses depending on how much you draw. Use the calculator above rather than guessing.

  • Swab the vial stopper, invert the vial, and draw slowly to avoid bubbles.
  • Tap out any air, then push back to the exact unit mark from the calculator.
  • Keep a log of the date reconstituted and the concentration written on the vial.
  • Start research protocols conservatively and reference published literature for the compound in question.
04

Storage & shelf life

Correct storage protects potency. Peptides degrade with heat, light and repeated temperature swings.

  • Lyophilised (unmixed): store in a freezer or fridge, away from light. Stable for many months.
  • Reconstituted: refrigerate at 2–8°C. Generally use within 3–4 weeks; because our water is bacteriostatic it resists microbial growth.
  • Never freeze a reconstituted vial — ice crystals damage the peptide.
  • Keep vials upright, labelled, and out of reach of anyone not conducting the research.
Important This guide is general research information, not medical or veterinary advice. All Vitae Labs products are for in-vitro laboratory research only and are not for human or animal consumption. Consult qualified professionals and published literature before designing any protocol.
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